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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/1028

Title: Separation of an isoenzyme of polyphenol oxidase from duranta plumieri by expanded bed chromatography
Authors: Roy, Ipsita
Sharma, Shweta
Gupta, Munishwar Nath
Keywords: polyphenol oxidase
expanded bed chromatography
duranta plumieri
enzyme latency
Issue Date: 2002
Citation: Protein Expression and Purification, 24(2), 181-187
Abstract: Aqueous extracts of seeds of Duranta plumieri were found to be rich in polyphenol oxidase activity. The anion-exchange chromatography of the crude extract on Streamline DEAE resolved the activity into three fractions. The major fraction (77% of the total activity) was further purified by treating it with concanavalin A-agarose in the batch mode. The enzyme preparation eluting with α-methylmannoside showed a single band on SDS-PAGE. The minimum molecular weight corresponded to 14,000 Da. The Km and Vmax of this isoenzyme were found to be 7.1 mM and 73.5 U ml−1 min−1 respectively. The kcat of this isoenzyme was calculated to be 8235 s−1. The isoenzyme also showed the phenomenon of latency and the activity could be enhanced by 196% on heating it at 55°C for 30 min.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/1028
Appears in Collections:Chemistry

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