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Please use this identifier to cite or link to this item:
http://hdl.handle.net/2074/1146
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Full metadata record
| DC Field | Value | Language |
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| contributor.author | Mondal, Kalyani | - |
| contributor.author | Sharma, Aparna | - |
| contributor.author | Gupta, Munishwar Nath | - |
| date.accessioned | 2006-01-04T13:28:19Z | - |
| date.available | 2006-01-04T13:28:19Z | - |
| date.issued | 2003 | - |
| identifier.citation | Protein Expression and Purification, 28(1), 190-195 | en |
| identifier.uri | http://eprint.iitd.ac.in/dspace/handle/2074/1146 | - |
| description.abstract | Starch-degrading enzymes glucoamylase (from Aspergillus niger), and pullulanase (from Bacillus acidopullulyticus) were purified using alginates (polysaccharides consisting of mannuronic acids and guluronic acids) by a recently developed technique called macroaffinity ligand-facilitated three-phase partitioning (MLFTPP). In this process, a crude preparation of the enzyme was mixed with alginate. On addition of appropriate amounts of ammonium sulfate and t-butanol, the alginate bound enzyme appeared as an interfacial precipitate between the lower aqueous and the upper t-butanol phase. Enzyme activity from this interfacial precipitate was recovered using 1 M maltose. Glucoamylase and pullulanase were purified 20- and 38-fold with 83% and 89% activity recovery, respectively. Both the purified preparations showed a single band on SDS–PAGE. | en |
| format.extent | 191701 bytes | - |
| format.mimetype | application/pdf | - |
| language.iso | en | en |
| subject | Alginates | en |
| subject | Smart polymers | en |
| subject | Glucoamylase | en |
| subject | Pullulanase | en |
| subject | Three-phase partitioning (TPP) | en |
| subject | Macroaffinity ligand-facilitated three-phase partitioning (MLFTPP) | en |
| title | Macroaffinity ligand-facilitated three-phase partitioning for purification of glucoamylase and pullulanase using alginate | en |
| type | Article | en |
| Appears in Collections: | Chemistry
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| mondalmac2003.pdf | | 187Kb | Adobe PDF | View/Open |
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