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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/1191

Title: κ-Carrageenan as a new smart macroaffinity ligand for the purification of pullulanase
Authors: Roy, Ipsita
Gupta, M N
Keywords: Pullulanase
Enzymes
Carrageenan
Issue Date: 2003
Citation: Journal of Chromatography A, 998(1-2), 103-108
Abstract: κ-Carrageenan is a polysaccharide from red seaweed which gets precipitated by K+ ions and dissolves again in water. This smart, K+-responsive polymer was found to selectively bind pullulanase activity from Bacillus acidopullulyticus. Gel filtration on Sephadex G-200 showed the formation of the polymer–pullulanase complex at the pre-precipitation stage. On the other hand, phospholipase D, an enzyme which did not co-precipitate with κ-carrageenan, did not form any complex with the polymer. Thus, K+ ions could be used to selectively precipitate the pullulanase activity. Then, 92% enzyme activity could be eluted with 1 M maltose solution. The single step protocol resulted in 50-fold purification, with a single band on sodium dodecylsulfate–polyacrylamide gel electrophoresis.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/1191
Appears in Collections:Chemistry

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