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http://hdl.handle.net/2074/1191
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| Title: | κ-Carrageenan as a new smart macroaffinity ligand for the purification of pullulanase |
| Authors: | Roy, Ipsita
Gupta, M N |
| Keywords: | Pullulanase
Enzymes
Carrageenan |
| Issue Date: | 2003 |
| Citation: | Journal of Chromatography A, 998(1-2), 103-108 |
| Abstract: | κ-Carrageenan is a polysaccharide from red seaweed which gets precipitated by K+ ions and dissolves again in water. This smart, K+-responsive polymer was found to selectively bind pullulanase activity from Bacillus acidopullulyticus. Gel filtration on Sephadex G-200 showed the formation of the polymer–pullulanase complex at the pre-precipitation stage. On the other hand, phospholipase D, an enzyme which did not co-precipitate with κ-carrageenan, did not form any complex with the polymer. Thus, K+ ions could be used to selectively precipitate the pullulanase activity. Then, 92% enzyme activity could be eluted with 1 M maltose solution. The single step protocol resulted in 50-fold purification, with a single band on sodium dodecylsulfate–polyacrylamide gel electrophoresis. |
| URI: | http://eprint.iitd.ac.in/dspace/handle/2074/1191 |
| Appears in Collections: | Chemistry
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