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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/1233

Title: κ-Carrageenan as a carrier in affinity precipitation of yeast alcohol dehydrogenase
Authors: Mondal, Kalyani
Roy, Ipsita
Gupta, Munishwar N
Keywords: Affinity precipitation
Alcohol dehydrogenase
Reversibly soluble–insoluble polymers
Protein purification
Issue Date: 2003
Citation: Protein Expression and Purification, 32(1), 151-160
Abstract: κ-Carrageenan is a non-toxic polymer from seaweeds, which becomes reversibly insoluble upon the addition of K+. Its conjugate with the dye, Cibacron Blue 3GA, was used to purify alcohol dehydrogenase from crude yeast extract by affinity precipitation. Response surface methodology was used to optimize conditions for affinity precipitation of the enzyme with the polymer–dye conjugate. Recovery of 58% of the enzyme activity with 13.6-fold purification was obtained.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/1233
Appears in Collections:Chemistry

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