EPrints@IIT Delhi : Item 2074/1233

Please use this identifier to cite or link to this item: http://hdl.handle.net/2074/1233

Full metadata record

DC Field	Value	Language
contributor.author	Mondal, Kalyani	-
contributor.author	Roy, Ipsita	-
contributor.author	Gupta, Munishwar N	-
date.accessioned	2006-01-24T05:57:10Z	-
date.available	2006-01-24T05:57:10Z	-
date.issued	2003	-
identifier.citation	Protein Expression and Purification, 32(1), 151-160	en
identifier.uri	http://eprint.iitd.ac.in/dspace/handle/2074/1233	-
description.abstract	κ-Carrageenan is a non-toxic polymer from seaweeds, which becomes reversibly insoluble upon the addition of K+. Its conjugate with the dye, Cibacron Blue 3GA, was used to purify alcohol dehydrogenase from crude yeast extract by affinity precipitation. Response surface methodology was used to optimize conditions for affinity precipitation of the enzyme with the polymer–dye conjugate. Recovery of 58% of the enzyme activity with 13.6-fold purification was obtained.	en
format.extent	489584 bytes	-
format.mimetype	application/pdf	-
language.iso	en	en
subject	Affinity precipitation	en
subject	Alcohol dehydrogenase	en
subject	κ-Carrageenan	en
subject	Reversibly soluble–insoluble polymers	en
subject	Protein purification	en
title	κ-Carrageenan as a carrier in affinity precipitation of yeast alcohol dehydrogenase	en
type	Article	en
Appears in Collections:	Chemistry

Files in This Item:

File	Description	Size	Format
mondalcar2003.pdf		478Kb	Adobe PDF	View/Open