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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/1233

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dc.contributor.authorMondal, Kalyani-
dc.contributor.authorRoy, Ipsita-
dc.contributor.authorGupta, Munishwar N-
dc.date.accessioned2006-01-24T05:57:10Z-
dc.date.available2006-01-24T05:57:10Z-
dc.date.issued2003-
dc.identifier.citationProtein Expression and Purification, 32(1), 151-160en
dc.identifier.urihttp://eprint.iitd.ac.in/dspace/handle/2074/1233-
dc.description.abstractκ-Carrageenan is a non-toxic polymer from seaweeds, which becomes reversibly insoluble upon the addition of K+. Its conjugate with the dye, Cibacron Blue 3GA, was used to purify alcohol dehydrogenase from crude yeast extract by affinity precipitation. Response surface methodology was used to optimize conditions for affinity precipitation of the enzyme with the polymer–dye conjugate. Recovery of 58% of the enzyme activity with 13.6-fold purification was obtained.en
dc.format.extent489584 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.subjectAffinity precipitationen
dc.subjectAlcohol dehydrogenaseen
dc.subjectκ-Carrageenanen
dc.subjectReversibly soluble–insoluble polymersen
dc.subjectProtein purificationen
dc.titleκ-Carrageenan as a carrier in affinity precipitation of yeast alcohol dehydrogenaseen
dc.typeArticleen
Appears in Collections:Chemistry

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