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Please use this identifier to cite or link to this item: http://hdl.handle.net/2074/1237

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contributor.authorSafarikova, M-
contributor.authorRoy, I-
contributor.authorGupta, M N-
contributor.authorSafarik, I-
date.accessioned2006-01-24T06:10:05Z-
date.available2006-01-24T06:10:05Z-
date.issued2003-
identifier.citationJournal of Biotechnology, 105(3), 255-260en
identifier.urihttp://eprint.iitd.ac.in/dspace/handle/2074/1237-
description.abstractSpherical magnetic alginate microparticles (25–60 μm in diameter) were prepared using the microemulsion system, with water-saturated 1-pentanol as the organic phase. The limited solubility of 1-pentanol in water enabled simple removal of the organic solvent from the prepared beads with water solution. The prepared alginate microparticles were used as magnetic affinity adsorbents for specific purification of α-amylases. Enzyme activity was eluted by 1.0 M maltose. α-Amylases from Bacillus amyloliquefaciens and porcine pancreatic acetone powder were purified 9- and 12-fold with 88 and 96% activity recovery, respectively.en
format.extent557919 bytes-
format.mimetypeapplication/pdf-
language.isoenen
subjectAlginateen
subjectFerrofluiden
subjectMagnetic fluiden
subjectMagnetic microparticlesen
subjectMagnetic nanoparticlesen
subjectAmylasesen
titleMagnetic alginate microparticles for purification of α-amylasesen
typeArticleen
Appears in Collections:Chemistry

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