DSpace
 

EPrints@IIT Delhi >
Faculty Research Publicatons  >
Chemistry >

Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/1237

Full metadata record

DC FieldValueLanguage
dc.contributor.authorSafarikova, M-
dc.contributor.authorRoy, I-
dc.contributor.authorGupta, M N-
dc.contributor.authorSafarik, I-
dc.date.accessioned2006-01-24T06:10:05Z-
dc.date.available2006-01-24T06:10:05Z-
dc.date.issued2003-
dc.identifier.citationJournal of Biotechnology, 105(3), 255-260en
dc.identifier.urihttp://eprint.iitd.ac.in/dspace/handle/2074/1237-
dc.description.abstractSpherical magnetic alginate microparticles (25–60 μm in diameter) were prepared using the microemulsion system, with water-saturated 1-pentanol as the organic phase. The limited solubility of 1-pentanol in water enabled simple removal of the organic solvent from the prepared beads with water solution. The prepared alginate microparticles were used as magnetic affinity adsorbents for specific purification of α-amylases. Enzyme activity was eluted by 1.0 M maltose. α-Amylases from Bacillus amyloliquefaciens and porcine pancreatic acetone powder were purified 9- and 12-fold with 88 and 96% activity recovery, respectively.en
dc.format.extent557919 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.subjectAlginateen
dc.subjectFerrofluiden
dc.subjectMagnetic fluiden
dc.subjectMagnetic microparticlesen
dc.subjectMagnetic nanoparticlesen
dc.subjectAmylasesen
dc.titleMagnetic alginate microparticles for purification of α-amylasesen
dc.typeArticleen
Appears in Collections:Chemistry

Files in This Item:

File Description SizeFormat
safarikovamag2003.pdf544.84 kBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback