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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/1237

Title: Magnetic alginate microparticles for purification of α-amylases
Authors: Safarikova, M
Roy, I
Gupta, M N
Safarik, I
Keywords: Alginate
Ferrofluid
Magnetic fluid
Magnetic microparticles
Magnetic nanoparticles
Amylases
Issue Date: 2003
Citation: Journal of Biotechnology, 105(3), 255-260
Abstract: Spherical magnetic alginate microparticles (25–60 μm in diameter) were prepared using the microemulsion system, with water-saturated 1-pentanol as the organic phase. The limited solubility of 1-pentanol in water enabled simple removal of the organic solvent from the prepared beads with water solution. The prepared alginate microparticles were used as magnetic affinity adsorbents for specific purification of α-amylases. Enzyme activity was eluted by 1.0 M maltose. α-Amylases from Bacillus amyloliquefaciens and porcine pancreatic acetone powder were purified 9- and 12-fold with 88 and 96% activity recovery, respectively.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/1237
Appears in Collections:Chemistry

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