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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/1244

Title: Evaluation of a smart bioconjugate of pectinase for chitin hydrolysis
Authors: Roy, Ipsita
Sardar, Meryam
Gupta, Munishwar N
Keywords: Bioconversion
Chitin hydrolysis
Enzyme technology
Immobilized enzymes
Pectinase
Waste treatment
Issue Date: 2003
Citation: Biochemical Engineering Journal, 16(3), 329-335
Abstract: Pectinex™ Ultra SP-L was immobilized non-covalently on alginate. The smart (reversibly soluble–insoluble) bioconjugate showed 56% activity as compared to the equivalent free enzyme for hydrolysis of chitin. The bioconjugate showed a broader pH optimum while the temperature optimum remained unchanged, as compared to the free enzyme. The bioconjugate also showed enhanced thermal stability at 55 and 65 °C. The Km and Vmax values of the bioconjugate were 6 mg ml−1 and 3.4 nmol min−1, respectively. There was 55% reduction in the bioconjugate activity after first cycle. The reason behind this reduction was investigated and is briefly discussed.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/1244
Appears in Collections:Chemistry

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