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http://hdl.handle.net/2074/1329
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| Title: | Affinity precipitation of aspergillus niger pectinase by microwave-treated alginate |
| Authors: | Mondal, Kalyani
Mehta, Payal
Gupta, Munishwar N |
| Keywords: | Affinity precipitation
Affinity ligands
Alginate
Microwave pretreatment
Pectinase
Protein purification |
| Issue Date: | 2004 |
| Citation: | Protein Expression and Purification, 33(1), 104-109 |
| Abstract: | Affinity precipitation is a simple, single plate separation process in which the complex of a smart macroaffinity ligand with the target protein (from a crude broth) can be selectively precipitated by application of a suitable stimulus. Alginate is a copolymer of guluronic acid and mannuronic acid residues and precipitates with Ca2+ ions. It was found to bind to pectinase present in a commercial preparation of Aspergillus niger, Pectinex Ultra-SPL. Microwave pretreatment of alginate at 75 °C was found to enhance the selectivity of the affinity precipitation. Using microwave-treated alginate, 83% of the enzyme activity with 20-fold purification could be recovered. SDS–PAGE upon silver staining confirmed the enhanced selectivity of affinity precipitation when microwave-treated alginate was used. |
| URI: | http://eprint.iitd.ac.in/dspace/handle/2074/1329 |
| Appears in Collections: | Chemistry
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| mondalaff2004.pdf | | 299Kb | Adobe PDF | View/Open |
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