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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/1355

Title: Obtaining higher transesterification rates with subtilisin carlsberg in nonaqueous media
Authors: Roy, Ipsita
Sharma, Aparna
Gupta, Munishwar N
Keywords: Cryoprotectant
Subtilisin Carlsberg
Three phase partitioning
Issue Date: 2004
Citation: Bioorganic & Medicinal Chemistry Letters, 14(4), 887-889
Abstract: Three phase partitioning (protein precipitate obtained as an interfacial layer between lower aqueous and upper t-butanol phases, formed by the addition of ammonium sulphate and t-butanol to the aqueous solution of protein) followed by lyophilization in the presence of two-component excipient resulted in 400–480× increases in transesterification activity of lyophilized powders of subtilisin Carlsberg, depending on the solvent. The three phase partitioned enzyme, ‘dried’ by washing with butanol, gave 3–4× higher rates (depending on the solvent used) than the enzyme preparation dried by lyophilization in the presence of two-component excipient system.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/1355
Appears in Collections:Chemistry

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