EPrints@IIT Delhi : Item 2074/1355

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DC Field	Value	Language
contributor.author	Roy, Ipsita	-
contributor.author	Sharma, Aparna	-
contributor.author	Gupta, Munishwar N	-
date.accessioned	2006-02-21T04:47:06Z	-
date.available	2006-02-21T04:47:06Z	-
date.issued	2004	-
identifier.citation	Bioorganic & Medicinal Chemistry Letters, 14(4), 887-889	en
identifier.uri	http://eprint.iitd.ac.in/dspace/handle/2074/1355	-
description.abstract	Three phase partitioning (protein precipitate obtained as an interfacial layer between lower aqueous and upper t-butanol phases, formed by the addition of ammonium sulphate and t-butanol to the aqueous solution of protein) followed by lyophilization in the presence of two-component excipient resulted in 400–480× increases in transesterification activity of lyophilized powders of subtilisin Carlsberg, depending on the solvent. The three phase partitioned enzyme, ‘dried’ by washing with butanol, gave 3–4× higher rates (depending on the solvent used) than the enzyme preparation dried by lyophilization in the presence of two-component excipient system.	en
format.extent	136679 bytes	-
format.mimetype	application/pdf	-
language.iso	en	en
subject	Cryoprotectant	en
subject	Lyoprotectant	en
subject	Subtilisin Carlsberg	en
subject	Three phase partitioning	en
subject	Transesterification	en
title	Obtaining higher transesterification rates with subtilisin carlsberg in nonaqueous media	en
type	Article	en
Appears in Collections:	Chemistry

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