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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/1403

Title: Three-phase partitioning for simultaneous renaturation and partial purification of Aspergillus niger xylanase
Authors: Roy, Ipsita
Sharma, Aparna
Gupta, Munishwar Nath
Keywords: Inclusion body
Protein purification
Protein refolding
Three-phase partitioning
Xylanase
Issue Date: 2004
Citation: Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics, 1698(1), 107-110
Abstract: Three-phase partitioning (TPP) is carried out by mixing ammonium sulfate and t-butanol to obtain organic phase, interfacial precipitate and aqueous phase. It is shown that TPP of an 8 M urea/100 mM dithiothreitol-denatured xylanase preparation resulted in simultaneous renaturation and purification. This integrated novel approach gave recovery of 93% enzyme activity with 21-fold purification. The implications of this in the context of recovering activity from inclusion bodies are discussed.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/1403
Appears in Collections:Chemistry

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