|
EPrints@IIT Delhi >
Faculty Research Publicatons >
Chemistry >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/2074/1410
|
| Title: | Purification of green fluorescent protein overexpressed by a mutant recombinant Escherichia coli |
| Authors: | Jain, Sulakshana
Teotia, Sunita
Gupta, Munishwar N |
| Keywords: | Green fluorescent protein
Protein overexpression
Protein purification
Selective precipitation
Two-phase aqueous extraction |
| Issue Date: | 2004 |
| Citation: | Protein Expression and Purification, 36(1), 76-81 |
| Abstract: | Green fluorescent protein was purified from sonicated recombinant Escherichia coli and its mutant obtained after exposure to UV light. The latter overexpresses green fluorescent protein. The two-step procedure consisted of a two-phase aqueous extraction with PEG/salt and precipitation of the proteins from PEG phase by free Zn2+. The recoveries of green fluorescent protein were 73 and 83% in the cases of recombinant E. coli and its mutant, respectively. The corresponding fold purifications were 24 and 9, respectively. In both cases, the purified protein showed a single band on SDS–PAGE corresponding to 28 kDa. |
| URI: | http://eprint.iitd.ac.in/dspace/handle/2074/1410 |
| Appears in Collections: | Chemistry
|
Files in This Item:
| File |
Description |
Size | Format |
|---|
| jainpuri2004.pdf | | 186Kb | Adobe PDF | View/Open |
|
Show full item record
All items in DSpace are protected by copyright, with all rights reserved.
|
