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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/1410

Title: Purification of green fluorescent protein overexpressed by a mutant recombinant Escherichia coli
Authors: Jain, Sulakshana
Teotia, Sunita
Gupta, Munishwar N
Keywords: Green fluorescent protein
Protein overexpression
Protein purification
Selective precipitation
Two-phase aqueous extraction
Issue Date: 2004
Citation: Protein Expression and Purification, 36(1), 76-81
Abstract: Green fluorescent protein was purified from sonicated recombinant Escherichia coli and its mutant obtained after exposure to UV light. The latter overexpresses green fluorescent protein. The two-step procedure consisted of a two-phase aqueous extraction with PEG/salt and precipitation of the proteins from PEG phase by free Zn2+. The recoveries of green fluorescent protein were 73 and 83% in the cases of recombinant E. coli and its mutant, respectively. The corresponding fold purifications were 24 and 9, respectively. In both cases, the purified protein showed a single band on SDS–PAGE corresponding to 28 kDa.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/1410
Appears in Collections:Chemistry

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