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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/1410

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dc.contributor.authorJain, Sulakshana-
dc.contributor.authorTeotia, Sunita-
dc.contributor.authorGupta, Munishwar N-
dc.date.accessioned2006-02-23T04:23:03Z-
dc.date.available2006-02-23T04:23:03Z-
dc.date.issued2004-
dc.identifier.citationProtein Expression and Purification, 36(1), 76-81en
dc.identifier.urihttp://eprint.iitd.ac.in/dspace/handle/2074/1410-
dc.description.abstractGreen fluorescent protein was purified from sonicated recombinant Escherichia coli and its mutant obtained after exposure to UV light. The latter overexpresses green fluorescent protein. The two-step procedure consisted of a two-phase aqueous extraction with PEG/salt and precipitation of the proteins from PEG phase by free Zn2+. The recoveries of green fluorescent protein were 73 and 83% in the cases of recombinant E. coli and its mutant, respectively. The corresponding fold purifications were 24 and 9, respectively. In both cases, the purified protein showed a single band on SDS–PAGE corresponding to 28 kDa.en
dc.format.extent191015 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.subjectGreen fluorescent proteinen
dc.subjectProtein overexpressionen
dc.subjectProtein purificationen
dc.subjectSelective precipitationen
dc.subjectTwo-phase aqueous extractionen
dc.titlePurification of green fluorescent protein overexpressed by a mutant recombinant Escherichia colien
dc.typeArticleen
Appears in Collections:Chemistry

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