EPrints@IIT Delhi : Item 2074/1410

Please use this identifier to cite or link to this item: http://hdl.handle.net/2074/1410

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DC Field	Value	Language
contributor.author	Jain, Sulakshana	-
contributor.author	Teotia, Sunita	-
contributor.author	Gupta, Munishwar N	-
date.accessioned	2006-02-23T04:23:03Z	-
date.available	2006-02-23T04:23:03Z	-
date.issued	2004	-
identifier.citation	Protein Expression and Purification, 36(1), 76-81	en
identifier.uri	http://eprint.iitd.ac.in/dspace/handle/2074/1410	-
description.abstract	Green fluorescent protein was purified from sonicated recombinant Escherichia coli and its mutant obtained after exposure to UV light. The latter overexpresses green fluorescent protein. The two-step procedure consisted of a two-phase aqueous extraction with PEG/salt and precipitation of the proteins from PEG phase by free Zn2+. The recoveries of green fluorescent protein were 73 and 83% in the cases of recombinant E. coli and its mutant, respectively. The corresponding fold purifications were 24 and 9, respectively. In both cases, the purified protein showed a single band on SDS–PAGE corresponding to 28 kDa.	en
format.extent	191015 bytes	-
format.mimetype	application/pdf	-
language.iso	en	en
subject	Green fluorescent protein	en
subject	Protein overexpression	en
subject	Protein purification	en
subject	Selective precipitation	en
subject	Two-phase aqueous extraction	en
title	Purification of green fluorescent protein overexpressed by a mutant recombinant Escherichia coli	en
type	Article	en
Appears in Collections:	Chemistry

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