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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/2410

Title: Quantitative reflectance haemoglobinometry
Authors: Guha, S K
Koul, V
Anand, S
Keywords: haemoglobinometry
reflection measurement
saponin
sodium nitrite
Issue Date: 1992
Citation: Measurement, 10(1), 43-48p.
Abstract: Conventional haemoglobinometry based upon optical transmission characterisation involves volumetric measurements and chemical processing in liquid media, thus making the procedures cumbersome. Reflection measurements being simple in practice, the now obsolete Tallqvist's reflection and visual colour matching technique has been scientifically reassessed. Three factors identified to be contributing to variable results from this method are: inadequate haemolysis of red blood cells; oxygenation variations; and no blood volume control. Based upon this identification, a new technique has been formulated which overcomes all the limitations of the Tallqvist's method. Consistent results are obtained even without volumetric control. Saponin and sodium nitrite impregnated into filter paper induce haemolysis and conversion of blood to a stable form independent of oxygen concentration. Thus the sources of errors are removed. Since reflectance measurement at a narrow wavelength range is adequate, the approach can lead to a simple haemoglobinometer.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/2410
Appears in Collections:Biomedical Engineering [CBME]

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