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Please use this identifier to cite or link to this item:
http://hdl.handle.net/2074/2413
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Full metadata record
| DC Field | Value | Language |
|---|
| contributor.author | A Kumar | - |
| contributor.author | Gupta, M N | - |
| date.accessioned | 2007-01-12T09:18:59Z | - |
| date.available | 2007-01-12T09:18:59Z | - |
| date.issued | 1994 | - |
| identifier.citation | Journal of Biotechnology, 37(2), 185-189p. | en |
| identifier.uri | http://eprint.iitd.ac.in/dspace/handle/2074/2413 | - |
| description.abstract | Soybean trypsin inhibitor linked to Eudragit S-100 was used for the affinity precipitation of trypsin. Polymer and ligand concentrations used in conjugate preparation showed remarkable effect on the trypsin recovery. Trypsin precipitation efficiency amounted to 89% and recovery was 74%. The final purification of relatively crude commercial trypsin resulted in 1.85-fold purification. The SDS-PAGE analysis indicated significant purification. The precipitated enzyme activity was around 96% and recovered enzyme activity was 83%. | en |
| format.extent | 152316 bytes | - |
| format.mimetype | application/pdf | - |
| language.iso | en | en |
| subject | affinity precipitation | en |
| subject | benzamidine | en |
| subject | eudragit S-100 | en |
| subject | soybean trypsin inhibitor | en |
| subject | trypsin | en |
| title | Affinity precipitation of trypsin with soybean trypsin inhibitor linked Eudragit S-100 | en |
| type | Article | en |
| Appears in Collections: | Chemistry
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Files in This Item:
| File |
Description |
Size | Format |
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| kumaraff94.pdf | | 148Kb | Adobe PDF | View/Open |
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