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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/2413

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dc.contributor.authorA Kumar-
dc.contributor.authorGupta, M N-
dc.date.accessioned2007-01-12T09:18:59Z-
dc.date.available2007-01-12T09:18:59Z-
dc.date.issued1994-
dc.identifier.citationJournal of Biotechnology, 37(2), 185-189p.en
dc.identifier.urihttp://eprint.iitd.ac.in/dspace/handle/2074/2413-
dc.description.abstractSoybean trypsin inhibitor linked to Eudragit S-100 was used for the affinity precipitation of trypsin. Polymer and ligand concentrations used in conjugate preparation showed remarkable effect on the trypsin recovery. Trypsin precipitation efficiency amounted to 89% and recovery was 74%. The final purification of relatively crude commercial trypsin resulted in 1.85-fold purification. The SDS-PAGE analysis indicated significant purification. The precipitated enzyme activity was around 96% and recovered enzyme activity was 83%.en
dc.format.extent152316 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.subjectaffinity precipitationen
dc.subjectbenzamidineen
dc.subjecteudragit S-100en
dc.subjectsoybean trypsin inhibitoren
dc.subjecttrypsinen
dc.titleAffinity precipitation of trypsin with soybean trypsin inhibitor linked Eudragit S-100en
dc.typeArticleen
Appears in Collections:Chemistry

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