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http://hdl.handle.net/2074/2413
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| Title: | Affinity precipitation of trypsin with soybean trypsin inhibitor linked Eudragit S-100 |
| Authors: | A Kumar
Gupta, M N |
| Keywords: | affinity precipitation
benzamidine
eudragit S-100
soybean trypsin inhibitor
trypsin |
| Issue Date: | 1994 |
| Citation: | Journal of Biotechnology, 37(2), 185-189p. |
| Abstract: | Soybean trypsin inhibitor linked to Eudragit S-100 was used for the affinity precipitation of trypsin. Polymer and ligand concentrations used in conjugate preparation showed remarkable effect on the trypsin recovery. Trypsin precipitation efficiency amounted to 89% and recovery was 74%. The final purification of relatively crude commercial trypsin resulted in 1.85-fold purification. The SDS-PAGE analysis indicated significant purification. The precipitated enzyme activity was around 96% and recovered enzyme activity was 83%. |
| URI: | http://eprint.iitd.ac.in/dspace/handle/2074/2413 |
| Appears in Collections: | Chemistry
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| kumaraff94.pdf | | 148Kb | Adobe PDF | View/Open |
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