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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/2413

Title: Affinity precipitation of trypsin with soybean trypsin inhibitor linked Eudragit S-100
Authors: A Kumar
Gupta, M N
Keywords: affinity precipitation
benzamidine
eudragit S-100
soybean trypsin inhibitor
trypsin
Issue Date: 1994
Citation: Journal of Biotechnology, 37(2), 185-189p.
Abstract: Soybean trypsin inhibitor linked to Eudragit S-100 was used for the affinity precipitation of trypsin. Polymer and ligand concentrations used in conjugate preparation showed remarkable effect on the trypsin recovery. Trypsin precipitation efficiency amounted to 89% and recovery was 74%. The final purification of relatively crude commercial trypsin resulted in 1.85-fold purification. The SDS-PAGE analysis indicated significant purification. The precipitated enzyme activity was around 96% and recovered enzyme activity was 83%.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/2413
Appears in Collections:Chemistry

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