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http://hdl.handle.net/2074/2546
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| Title: | Enantioselective transacetylation of (R,S)-β-citronellol by propanol rinsed immobilized Rhizomucor miehei lipase |
| Authors: | Majumder, Abir B
Shah, Shweta
Gupta, Munishwar N |
| Keywords: | Kinetic resolution
Enzyme
Water molecules
Organic compounds |
| Issue Date: | 2007 |
| Citation: | Chemistry Central Journal 2007, 1(10), 1-5p. |
| Abstract: | Background: Use of enzymes in low water media is now widely used for synthesis and kineticresolution of organic compounds. The frequently used enzyme form is the freeze-dried powders.It has been shown earlier that removal of water molecules from enzyme by rinsing with n-propanolgives preparation (PREP) which show higher activity in low water media. The present workevaluates PREP of the lipase (from Rhizomucor miehei) for kinetic resolution of (R,S)-β-citronellol.The acylating agent was vinyl acetate and the reaction was carried out in solvent free media.Results: The PREP, with 0.75% (v/v, reaction media) water, was indeed found to be more efficientand gave 95% conversion to the ester. Using this PREP, with no added water, 90% ee for (R)-(+)-β-citronellyl acetate at 45% conversion (E = 42) was obtained in 4 h. The control with freeze-driedenzyme, with zero water content, gave 78% ee at 30% conversion (E = 13). FT-IR analysis showedthat PREP had retained the α-helical content of the enzy... |
| URI: | http://eprint.iitd.ac.in/dspace/handle/2074/2546 |
| Appears in Collections: | Chemistry
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| Enanti2007.pdf | | 988Kb | Adobe PDF | View/Open |
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