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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/2546

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dc.contributor.authorMajumder, Abir B-
dc.contributor.authorShah, Shweta-
dc.contributor.authorGupta, Munishwar N-
dc.date.accessioned2007-08-13T07:55:23Z-
dc.date.available2007-08-13T07:55:23Z-
dc.date.issued2007-
dc.identifier.citationChemistry Central Journal 2007, 1(10), 1-5p.en
dc.identifier.urihttp://eprint.iitd.ac.in/dspace/handle/2074/2546-
dc.description.abstractBackground: Use of enzymes in low water media is now widely used for synthesis and kineticresolution of organic compounds. The frequently used enzyme form is the freeze-dried powders.It has been shown earlier that removal of water molecules from enzyme by rinsing with n-propanolgives preparation (PREP) which show higher activity in low water media. The present workevaluates PREP of the lipase (from Rhizomucor miehei) for kinetic resolution of (R,S)-β-citronellol.The acylating agent was vinyl acetate and the reaction was carried out in solvent free media.Results: The PREP, with 0.75% (v/v, reaction media) water, was indeed found to be more efficientand gave 95% conversion to the ester. Using this PREP, with no added water, 90% ee for (R)-(+)-β-citronellyl acetate at 45% conversion (E = 42) was obtained in 4 h. The control with freeze-driedenzyme, with zero water content, gave 78% ee at 30% conversion (E = 13). FT-IR analysis showedthat PREP had retained the α-helical content of the enzyme. On the other hand, freeze-driedenzyme showed considerable loss in the α-helical content.Conclusion: The results show that PREP may be a superior biocatalyst for enantioselectiveconversion by enzymes in low-water media.en
dc.format.extent1012103 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.subjectKinetic resolutionen
dc.subjectEnzymeen
dc.subjectWater moleculesen
dc.subjectOrganic compoundsen
dc.titleEnantioselective transacetylation of (R,S)-β-citronellol by propanol rinsed immobilized Rhizomucor miehei lipaseen
dc.typeArticleen
Appears in Collections:Chemistry

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