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http://hdl.handle.net/2074/281
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| Title: | Purification of peanut lectin using guar gum as an affinity ligand |
| Authors: | Tyagi, Rem
Agarwal, Ritu
Gupta, M N |
| Keywords: | Affmity precipitation
Alginate
Galactose specific lectin
Peanut lectin
Guar gum
Protein purification |
| Issue Date: | 1996 |
| Publisher: | Ekevier Science |
| Citation: | Journal of Biotechnology 46 , 79-83 |
| Abstract: | Affinity precipitation is a convenient purification approach which combines the advantages of precipitation with selectivity of affinity interactions. The main limitation of the technique at present is the cost of affinity ligands and matrix. In this work, peanut lectin was isolated by affinity precipitation using guar gum linked alginate. Both matrix as well as
affinity ligand were inexpensive in nature. The recovered lectin (46 mg per 100 g seeds) urified by this single-step procedure appeared as a single band of M, 29000 on SDS- olyacrylamide gel electrophoresis. |
| URI: | http://eprint.iitd.ac.in/dspace/handle/2074/281 |
| Appears in Collections: | Chemistry
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| tyagipur96.pdf | | 466Kb | Adobe PDF | View/Open |
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