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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/281

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dc.contributor.authorTyagi, Rem-
dc.contributor.authorAgarwal, Ritu-
dc.contributor.authorGupta, M N-
dc.identifier.citationJournal of Biotechnology 46 , 79-83en
dc.description.abstractAffinity precipitation is a convenient purification approach which combines the advantages of precipitation with selectivity of affinity interactions. The main limitation of the technique at present is the cost of affinity ligands and matrix. In this work, peanut lectin was isolated by affinity precipitation using guar gum linked alginate. Both matrix as well as affinity ligand were inexpensive in nature. The recovered lectin (46 mg per 100 g seeds) urified by this single-step procedure appeared as a single band of M, 29000 on SDS- olyacrylamide gel electrophoresis.en
dc.format.extent482145 bytes-
dc.publisherEkevier Scienceen
dc.subjectAffmity precipitationen
dc.subjectGalactose specific lectinen
dc.subjectPeanut lectinen
dc.subjectGuar gumen
dc.subjectProtein purificationen
dc.titlePurification of peanut lectin using guar gum as an affinity liganden
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