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| Title: | Productivity enhancement in L-sorbose fermentation using oxygen vector |
| Authors: | Giridhar, R
Srivastava, A K |
| Keywords: | Vitamin-C
Acetobacter suboxydans
D-sorbitol
L-sorbose
Fed-batch fermentation
Oxygen vector |
| Issue Date: | 2000 |
| Citation: | Enzyme and microbial technology, 27, 537-541 |
| Abstract: | Sorbose, an intermediate of Vitamin-C is produced by biological oxidation of sorbitol by Acetobacter suboxydans. The utility of oxygen vector (N-hexadecane) in enhancing the sorbose accumulation and its productivity was examined for sorbitol to sorbose bioconversion process. Shake flask fermentations were conducted using 1% to 6% (v/v) N-hexadecane. Higher sorbose production was observed in shake
flask containing 1–4% N-hexadecane as compared to shake flasks without N-hexadecane. A maximum of 82.12 kgm23 sorbose accumulated in 24 h by the addition of 4% N-hexadecane as against 64.83 kgm23 sorbose without the addition of N-hexadecane. However, the concentration of sorbose produced in the same time decreased when 5% and 6% N-hexadecane were used. Batch sorbose fermentation conducted using 4% N-hexadecane demonstrated decrease in the process time from 14 h to 12 h. The productivity increased from 14.3
kgm23 h21 to 16.7 kgm23 h21 when 4% N-hexadecane was used. Fed-batch fermentation usin... |
| URI: | http://eprint.iitd.ac.in/dspace/handle/2074/393 |
| Appears in Collections: | Biochemical Engg. and Biotechnology
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| giridharpro2000.pdf | | 1172Kb | Adobe PDF | View/Open |
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