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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/393

Title: Productivity enhancement in L-sorbose fermentation using oxygen vector
Authors: Giridhar, R
Srivastava, A K
Keywords: Vitamin-C
Acetobacter suboxydans
D-sorbitol
L-sorbose
Fed-batch fermentation
Oxygen vector
Issue Date: 2000
Citation: Enzyme and microbial technology, 27, 537-541
Abstract: Sorbose, an intermediate of Vitamin-C is produced by biological oxidation of sorbitol by Acetobacter suboxydans. The utility of oxygen vector (N-hexadecane) in enhancing the sorbose accumulation and its productivity was examined for sorbitol to sorbose bioconversion process. Shake flask fermentations were conducted using 1% to 6% (v/v) N-hexadecane. Higher sorbose production was observed in shake flask containing 1–4% N-hexadecane as compared to shake flasks without N-hexadecane. A maximum of 82.12 kgm23 sorbose accumulated in 24 h by the addition of 4% N-hexadecane as against 64.83 kgm23 sorbose without the addition of N-hexadecane. However, the concentration of sorbose produced in the same time decreased when 5% and 6% N-hexadecane were used. Batch sorbose fermentation conducted using 4% N-hexadecane demonstrated decrease in the process time from 14 h to 12 h. The productivity increased from 14.3 kgm23 h21 to 16.7 kgm23 h21 when 4% N-hexadecane was used. Fed-batch fermentation using 4% N-hexadecane was over in 20 h with a productivity of 15.9 kgm23 h21, and a sorbose accumulation of 311.68 kgm23 was obtained. The same fed-batch fermentation finished in 25 h when no N-hexadecane was used. A productivity of 12.6 kgm23 h21 and a final sorbose accumulation of 316 kgm23 were obtained.The increase in productivity and lower process time were achieved by the addition of N-hexadecane to the fermentation medium.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/393
Appears in Collections:Biochemical Engg. and Biotechnology

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