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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/395

Title: Biosynthesis of tannase and hydrolysis of tannins to gallic acid by aspergillus awamori — optimisation of process parameters
Authors: Seth, Malini
Chand, Subhash
Keywords: Tannase biosynthesis
Gallic acid accumulation
Optimisation
Response surface method
Aspergillus awamori
Issue Date: 2000
Citation: Process Biochemistry, 36, 39-44
Abstract: Tannin acyl hydrolase (tannase) is an industrially important enzyme produced by a large number of fungi. Tannic acid concentration, agitation speed and pH during the fermentation were identified as important process parameters effecting cell growth and enzyme synthesis by Aspergillus awamori. These parameters were optimised in a laboratory bioreactor by response surface methodology using Box and Behnken factorial design to determine the optimum conditions for enzyme production and gallic acid accumulation. Under optimum process conditions for enzyme synthesis, the fermentation run lasted 60 h with an initial tannic acid concentration of 35.0 g l, yielding biomass concentration of 7.13 g l containing 771 IU of intracellular tannase per gram dry cell weight and 19 g l of gallic acid. However, maximum gallic acid accumulation (40.3 g l) was obtained in 24 h with an initial substrate concentration of 45 g l.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/395
Appears in Collections:Biochemical Engg. and Biotechnology

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