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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/456

Title: Effect of cysteine on methionine production by a regulatory mutant of corynebacterium lilium
Authors: Dharmendra Kumar
Subramanian, Kartik
Bisaria, Virendra S
Sreekrishnan, T R
Gomes, James
Keywords: Glc6P
glucose-6-phosphate
Fru6P
fructose-6-phosphate
GaP
glyceroldehyde-3-phosphate
G3P
3 phosphoglycerate
PEP
phosphoenol pyruvate
Pyr
pyruvate
AcCoA
acetyl coenzyme A
OaA
oxaloacetate
Isocit
isocitrate
aKG
alpha keto glutarate
SucCoA
succinyl coenzyme A
Suc
succinate
Mal
malate
Asp
aspartate
O-Shs
orthoacetylhomoserine
Meth
methionine
Lys
lysine
Thr
threonine
Ribu5P
ribulose 5 phosphate
Rib5p
ribose 5 phosphate
Xyl5p
xylulose 5 phosphate
Sed7P
sedoheptulose 7 phosphate
E4P
erythrose 4 phosphate
ADP
adenosine diphosphate
NADH
reduced nicotinamide adenine dinucleotide
FADH
reduced flavin adenine dinucleotide
NAD
nicotinamide adenine dinucleotide
FAD
flavin adenine dinucleotide
ATP
adenosine triphosphate
Issue Date: 2005
Citation: Bioresource technology, 96, 287–294
Abstract: The production of methionine by submerged fermentation using a mutant strain of Corynebacterium lilium was studied to determine suitable conditions for obtaining high productivity. The mutant strain resistant to the methionine analogues ethionine,norleucine, methionine sulfoxide and methionine methylsulfonium chloride produced 2.34 g1 of methionine in minimal medium containing glucose as carbon source. The effect of cysteine on methionine production in a 15 l bioreactor was studied by supplementing cysteine intermittently during the course of fermentation. The addition of cysteine (0.75 g1 h1) every 2 h to the productionm medium increased the production of methionine to 3.39 g1. A metabolic flux analysis showed that during cysteine supplementation the ATP consumption reduced by 20%. It also showed that the increase in flux from phosphoenol pyruvate to oxaloacetate leads to higher methionine production. Results indicate that controlling the respiratory quotient close to 0.75 will produce the highest amount of methionine and that regulatory mutants also resistant to analogues of cysteine would be better methionine over producers.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/456
Appears in Collections:Biochemical Engg. and Biotechnology

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