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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/456

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dc.contributor.authorDharmendra Kumar-
dc.contributor.authorSubramanian, Kartik-
dc.contributor.authorBisaria, Virendra S-
dc.contributor.authorSreekrishnan, T R-
dc.contributor.authorGomes, James-
dc.date.accessioned2005-07-21T04:04:21Z-
dc.date.available2005-07-21T04:04:21Z-
dc.date.issued2005-
dc.identifier.citationBioresource technology, 96, 287–294en
dc.identifier.urihttp://eprint.iitd.ac.in/dspace/handle/2074/456-
dc.description.abstractThe production of methionine by submerged fermentation using a mutant strain of Corynebacterium lilium was studied to determine suitable conditions for obtaining high productivity. The mutant strain resistant to the methionine analogues ethionine,norleucine, methionine sulfoxide and methionine methylsulfonium chloride produced 2.34 g1 of methionine in minimal medium containing glucose as carbon source. The effect of cysteine on methionine production in a 15 l bioreactor was studied by supplementing cysteine intermittently during the course of fermentation. The addition of cysteine (0.75 g1 h1) every 2 h to the productionm medium increased the production of methionine to 3.39 g1. A metabolic flux analysis showed that during cysteine supplementation the ATP consumption reduced by 20%. It also showed that the increase in flux from phosphoenol pyruvate to oxaloacetate leads to higher methionine production. Results indicate that controlling the respiratory quotient close to 0.75 will produce the highest amount of methionine and that regulatory mutants also resistant to analogues of cysteine would be better methionine over producers.en
dc.format.extent314593 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.subjectGlc6Pen
dc.subjectglucose-6-phosphateen
dc.subjectFru6Pen
dc.subjectfructose-6-phosphateen
dc.subjectGaPen
dc.subjectglyceroldehyde-3-phosphateen
dc.subjectG3Pen
dc.subject3 phosphoglycerateen
dc.subjectPEPen
dc.subjectphosphoenol pyruvateen
dc.subjectPyren
dc.subjectpyruvateen
dc.subjectAcCoAen
dc.subjectacetyl coenzyme Aen
dc.subjectOaAen
dc.subjectoxaloacetateen
dc.subjectIsociten
dc.subjectisocitrateen
dc.subjectaKGen
dc.subjectalpha keto glutarateen
dc.subjectSucCoAen
dc.subjectsuccinyl coenzyme Aen
dc.subjectSucen
dc.subjectsuccinateen
dc.subjectMalen
dc.subjectmalateen
dc.subjectAspen
dc.subjectaspartateen
dc.subjectO-Shsen
dc.subjectorthoacetylhomoserineen
dc.subjectMethen
dc.subjectmethionineen
dc.subjectLysen
dc.subjectlysineen
dc.subjectThren
dc.subjectthreonineen
dc.subjectRibu5Pen
dc.subjectribulose 5 phosphateen
dc.subjectRib5pen
dc.subjectribose 5 phosphateen
dc.subjectXyl5pen
dc.subjectxylulose 5 phosphateen
dc.subjectSed7Pen
dc.subjectsedoheptulose 7 phosphateen
dc.subjectE4Pen
dc.subjecterythrose 4 phosphateen
dc.subjectADPen
dc.subjectadenosine diphosphateen
dc.subjectNADHen
dc.subjectreduced nicotinamide adenine dinucleotideen
dc.subjectFADHen
dc.subjectreduced flavin adenine dinucleotideen
dc.subjectNADen
dc.subjectnicotinamide adenine dinucleotideen
dc.subjectFADen
dc.subjectflavin adenine dinucleotideen
dc.subjectATPen
dc.subjectadenosine triphosphateen
dc.titleEffect of cysteine on methionine production by a regulatory mutant of corynebacterium liliumen
dc.typeArticleen
Appears in Collections:Biochemical Engg. and Biotechnology

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