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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/481

Title: Role of water structure on phase separation in polyelectrolyte-polyethyleneglycol based aqueous two phase systems
Authors: Gupta, Vandana
Nath, Sunil
Chand, Subash
Keywords: Polyelectrolyte
Phase separation
Aqueous two-phase system
Issue Date: 2002
Citation: Polymer, 43(11), 3387-3390
Abstract: Partitioning of proteins in aqueous two-phase system(ATPS)has emerged as one of the important downstream processing techneques in bioprocess technology.The phase separation behavior of polyelectrolyte-polythyleneglycol(PEG)based ATPS have been studied to elucidate the mechanism controlling phase behaivor.The effect of various inorganic salt additvies revealed the importants of water structure as a major factor controlling phase separation in these systems.Nitrate and pottassium(water structure breaking ions)elevated the binodial line while sulphate,phosphate and sodium(water structure making ions)depressed the binodial line in both polyacrylic acid-PEG as-well-as polyethylelimine-PEG based ATPS.The effect of increase in concentration of either of the constitunt ploymers in both systems(at constant salt concentration)alwayes to led a greater propensity towards phase separation.These results point to a mechanism in which salt-assited polymer- modified water structure inactions play a central role in phase separation in ATPS.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/481
Appears in Collections:Biochemical Engg. and Biotechnology

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