|
EPrints@IIT Delhi >
Faculty Research Publicatons >
Biochemical Engg. and Biotechnology >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/2074/481
|
| Title: | Role of water structure on phase separation in polyelectrolyte-polyethyleneglycol based aqueous two phase systems |
| Authors: | Gupta, Vandana
Nath, Sunil
Chand, Subash |
| Keywords: | Polyelectrolyte
Phase separation
Aqueous two-phase system |
| Issue Date: | 2002 |
| Citation: | Polymer, 43(11), 3387-3390 |
| Abstract: | Partitioning of proteins in aqueous two-phase system(ATPS)has emerged as one of the important downstream processing techneques in bioprocess technology.The phase separation behavior of polyelectrolyte-polythyleneglycol(PEG)based ATPS have been studied to elucidate the mechanism controlling phase behaivor.The effect of various inorganic salt additvies revealed the importants of water structure as a major factor controlling phase separation in these systems.Nitrate and pottassium(water structure breaking ions)elevated the binodial line while sulphate,phosphate and sodium(water structure making ions)depressed the binodial line in both polyacrylic acid-PEG as-well-as polyethylelimine-PEG based ATPS.The effect of increase in concentration of either of the constitunt ploymers in both systems(at constant salt concentration)alwayes to led a greater propensity towards phase separation.These results point to a mechanism in which salt-assited polymer- modified water structure inactions play a cen... |
| URI: | http://eprint.iitd.ac.in/dspace/handle/2074/481 |
| Appears in Collections: | Biochemical Engg. and Biotechnology
|
Files in This Item:
| File |
Description |
Size | Format |
|---|
| guptarol2002.pdf | | 1070Kb | Adobe PDF | View/Open |
|
Show full item record
All items in DSpace are protected by copyright, with all rights reserved.
|
