DSpace
 

EPrints@IIT Delhi >
Faculty Research Publicatons  >
Biochemical Engg. and Biotechnology >

Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/481

Full metadata record

DC FieldValueLanguage
dc.contributor.authorGupta, Vandana-
dc.contributor.authorNath, Sunil-
dc.contributor.authorChand, Subash-
dc.date.accessioned2005-07-26T04:08:18Z-
dc.date.available2005-07-26T04:08:18Z-
dc.date.issued2002-
dc.identifier.citationPolymer, 43(11), 3387-3390en
dc.identifier.urihttp://eprint.iitd.ac.in/dspace/handle/2074/481-
dc.description.abstractPartitioning of proteins in aqueous two-phase system(ATPS)has emerged as one of the important downstream processing techneques in bioprocess technology.The phase separation behavior of polyelectrolyte-polythyleneglycol(PEG)based ATPS have been studied to elucidate the mechanism controlling phase behaivor.The effect of various inorganic salt additvies revealed the importants of water structure as a major factor controlling phase separation in these systems.Nitrate and pottassium(water structure breaking ions)elevated the binodial line while sulphate,phosphate and sodium(water structure making ions)depressed the binodial line in both polyacrylic acid-PEG as-well-as polyethylelimine-PEG based ATPS.The effect of increase in concentration of either of the constitunt ploymers in both systems(at constant salt concentration)alwayes to led a greater propensity towards phase separation.These results point to a mechanism in which salt-assited polymer- modified water structure inactions play a central role in phase separation in ATPS.en
dc.format.extent1095884 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.subjectPolyelectrolyteen
dc.subjectPhase separationen
dc.subjectAqueous two-phase systemen
dc.titleRole of water structure on phase separation in polyelectrolyte-polyethyleneglycol based aqueous two phase systemsen
dc.typeArticleen
Appears in Collections:Biochemical Engg. and Biotechnology

Files in This Item:

File Description SizeFormat
guptarol2002.pdf1.07 MBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback