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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/503

Title: Necessity of a two-stage process for the production of azadirachtin-related limonoids in suspension cultures of azadirachta indica
Authors: Raval, Keyur N
Hellwig, Stephan
Prakash, Gunjan
Plasencia, Alfredo Romos
Srivastava, Ashok
Buchs, Jochen
Keywords: azadirachta indica
plant cell suspension culture
respiration activity
oxygen transfer rate
specific power consumption
limonoid
azadirachtin
Issue Date: 2003
Citation: Journal of Bioscience and Bioengineering, 96(1), 16-22
Abstract: The effect of major nutrients on growth and azadirachtin-related limonoids (AZRL) production in plant cell culture of Azadirachta in&a (neem) was studied with the objective to increase the yield of AZRL, one of the major group of pesticidal compounds found in intact neem trees. We report the novel online monitoring of plant cell respiration activities in a new parallel shake flask measuring device. Results obtained using three standard plant cell culture media showed non-growth-associated production characteristics for AZRL. These findings were supported by the oxygen uptake rate data. Further investigations on AZRL production in a modified MS medium with different concentrations of nitrogen and phosphorus sources resulted in 0.25 mgeg-dry weight of AZRL, compared to no detectable AZRL production in standard MS media. These characteristics suggest the necessity of a two-stage process for the production of AZRL in plant cell culture. Compared to the single-stage process, an almost twofold increase in the volumetric productivity of AZRL was achieved using the two-stage process.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/503
Appears in Collections:Biochemical Engg. and Biotechnology

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