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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/612

Title: Theoretical aspects of single pulse induced modulation of cell–cell interactions favoring iso-electrofusion
Authors: Biswas, Subrata
Guha, Sujoy K
Keywords: Model cell surface
Cell–cell interactions
Issue Date: 1999
Citation: Bioelectrochemistry and Bioenergetics, 48(2), 423–429
Abstract: A single pulse-mediated electrofusion of freely suspending widely separated cells could reduce steps in the protocol. Prediction of such electrofusion is still unsolved. In pre-pulse condition, quantitative estimation with three-layered model cell surface reveals electrostatic repulsion due to negative surface charge is the major hindrance to membrane-to-membrane contact. On theoretical grounds we predict that designed single high voltage pulsing on widely separated model cell surface can counteract the non-specific repulsive interaction and favor approach of two apposed membranes. But hydrodynamic modulation of pulse exposed cell surface interaction can hamper approach of membranes contact when cells are held at a gap of more than-450 A.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/612
Appears in Collections:Biomedical Engineering [CBME]

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