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http://hdl.handle.net/2074/612
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| Title: | Theoretical aspects of single pulse induced modulation of cell–cell interactions favoring iso-electrofusion |
| Authors: | Biswas, Subrata
Guha, Sujoy K |
| Keywords: | Model cell surface
Iso-electrofusion
Cell–cell interactions |
| Issue Date: | 1999 |
| Citation: | Bioelectrochemistry and Bioenergetics, 48(2), 423–429 |
| Abstract: | A single pulse-mediated electrofusion of freely suspending widely separated cells could reduce steps in the protocol. Prediction of such electrofusion is still unsolved. In pre-pulse condition, quantitative estimation with three-layered model cell surface reveals electrostatic repulsion due to negative surface charge is the major hindrance to membrane-to-membrane contact. On theoretical grounds we predict that designed single high voltage pulsing on widely separated model cell surface can counteract the non-specific repulsive
interaction and favor approach of two apposed membranes. But hydrodynamic modulation of pulse exposed cell surface interaction can hamper approach of membranes contact when cells are held at a gap of more than-450 A. |
| URI: | http://eprint.iitd.ac.in/dspace/handle/2074/612 |
| Appears in Collections: | Biomedical Engineering [CBME]
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| biswasthe1999.pdf | | 212Kb | Adobe PDF | View/Open |
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