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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/616

Title: Membrane microextension: a possible mechanism for establishing molecular contact in electrofusion
Authors: Biswas, Subrata
Guha, Sujoy K.
Keywords: Membrane bump
Electrofusion
Membrane microextension
Morphological perturbation
Membrane contact
Issue Date: 1999
Citation: Bioelectrochemistry and Bioenergetics, 48(2), 435–440
Abstract: True cell membrane contact is an essential condition for electro-pulsed cell fusion, but initial morphological perturbation leading to true contact is still not clear. Dielectrophoresis mediated compression and fusogenic pulse induced compaction of cells led to rapid merger of tight membranes, and deprived direct microscopic view of surface membrane perturbation. Freely suspending cells with large and different cell–cell gaps may proceed to electrofusion with perturbed membrane and initiates fusion events at different time. These pulsed exposed cells can be used for capturing changes in the membrane surface and early electrofusion events. Early stage of fusion of freely suspended intact human erythrocytes exposed to single exponential decay pulse was studied by scanning electron microscopy SEM .Field pulse induces small membrane bumps. Interaction of bumps on adjacent membranes lead to true membrane contact and form bridges between the membranes as microextension, combining both membranes into a topologically single structure. Some fusion products showed expanded fusion zones, which suggest indication of open lumen at contact area.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/616
Appears in Collections:Biomedical Engineering [CBME]

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