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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/657

Title: A continuous process for production of d-ribulose-1,5-bisphosphate from d-glucose
Authors: Bhattacharya, Sumana
Nayaka, Amiya
Gomes, James
Bhattacharya, Sanjoy K
Keywords: RuBP production
Carbon dioxide fixation start-up
Immobilized enzymes
Issue Date: 2004
Citation: Biochemical Engineering Journal, 19(3), 229–235
Abstract: A continuous bioprocess for production of d-ribulose-1,5-bisphosphate (RuBP) using d-glucose as starting material is described. Based on ideal plug-flow reactor modeling using kinetic parameters, a series of plug-flow reactors were designed. A cohort of enzymes namely,hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase (6PGDH), ribose-5-phosphate isomerase (RPI) and phosphoribulokinase, purified, immobilized and resident in five successive plug-flow reactors enables a series of conversion resulting in an overall 60% yield of RuBP from d-glucose. Continuous conversion using this series of reactor provides a convenient route to produce RuBP in large quantities that could be used as starting material in other bioprocesses.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/657
Appears in Collections:Biochemical Engg. and Biotechnology

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