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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/747

Title: Exploiting unusual affinity of usual polysaccharides for separation of enzymes on fluidized beds
Authors: Roy, Ipsita
Sardar, Meryam
Gupta, Munishwar N
Keywords: Fluidized bed affinity chromatography
Polysaccharides
Bioseparation
Carbohydrases
a-amylase
cellulase
Issue Date: 2000
Citation: Enzyme and Microbial Technology, 27(1-2), 53–65
Abstract: Two polysaccharides, alginate and chitosan, showed unusual affinity and bound a-amylase (from various sources) and Aspergillus niger cellulase, respectively. The beads prepared from these polymers were successfully used for the purification of the respective enzymes by fluidized bed affinity chromatography. a-amylase from wheat germ could be purified by 58-fold with about 90% recovery of activity.Aspergillus niger cellulase, on the other hand, was purified by 30-fold with 80% recovery of enzyme activity. Both purified preparations show single band on SDS-PAGE.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/747
Appears in Collections:Chemistry

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