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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/747

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dc.contributor.authorRoy, Ipsita-
dc.contributor.authorSardar, Meryam-
dc.contributor.authorGupta, Munishwar N-
dc.date.accessioned2005-08-13T06:32:42Z-
dc.date.available2005-08-13T06:32:42Z-
dc.date.issued2000-
dc.identifier.citationEnzyme and Microbial Technology, 27(1-2), 53–65en
dc.identifier.urihttp://eprint.iitd.ac.in/dspace/handle/2074/747-
dc.description.abstractTwo polysaccharides, alginate and chitosan, showed unusual affinity and bound a-amylase (from various sources) and Aspergillus niger cellulase, respectively. The beads prepared from these polymers were successfully used for the purification of the respective enzymes by fluidized bed affinity chromatography. a-amylase from wheat germ could be purified by 58-fold with about 90% recovery of activity.Aspergillus niger cellulase, on the other hand, was purified by 30-fold with 80% recovery of enzyme activity. Both purified preparations show single band on SDS-PAGE.en
dc.format.extent2398716 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.subjectFluidized bed affinity chromatographyen
dc.subjectPolysaccharidesen
dc.subjectBioseparationen
dc.subjectCarbohydrasesen
dc.subjecta-amylaseen
dc.subjectcellulaseen
dc.titleExploiting unusual affinity of usual polysaccharides for separation of enzymes on fluidized bedsen
dc.typeArticleen
Appears in Collections:Chemistry

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