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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/765

Title: Purification of wheat germ amylase by precipitation
Authors: Sharma, Aparna
Sharma, Shweta
Gupta, M N
Keywords: amylase
amylase from wheat germ
purification
wheat
Issue Date: 2000
Citation: Protein Expression and Purification, 18(1), 111–114
Abstract: Amylase from various sources was found to bind alginate in free solution. The alginate–enzyme complex could be precipitated with Ca2+. The enzyme activity could be recovered by dissolving the precipitate in 1 M maltose and precipitating alginate alone by addition of Ca2+. Based upon these observations, a-amylase from wheat germ was purified with 68-fold purification and 72% recovery. The molecular weight estimated by SDS–PAGE was 18 kDa. The method also worked equally well with a-amylase for the whole wheat seed. The latter enzyme could be purified 54- fold with 70% activity recovery. The molecular weight of this second enzyme was estimated to be 45 kDa by SDS–PAGE.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/765
Appears in Collections:Chemistry

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