|
EPrints@IIT Delhi >
Faculty Research Publicatons >
Chemistry >
Please use this identifier to cite or link to this item:
http://hdl.handle.net/2074/765
|
Full metadata record
| DC Field | Value | Language |
|---|
| contributor.author | Sharma, Aparna | - |
| contributor.author | Sharma, Shweta | - |
| contributor.author | Gupta, M N | - |
| date.accessioned | 2005-08-13T07:41:41Z | - |
| date.available | 2005-08-13T07:41:41Z | - |
| date.issued | 2000 | - |
| identifier.citation | Protein Expression and Purification, 18(1), 111–114 | en |
| identifier.uri | http://eprint.iitd.ac.in/dspace/handle/2074/765 | - |
| description.abstract | Amylase from various sources was found to bind alginate in free solution. The alginate–enzyme complex could be precipitated with Ca2+. The enzyme activity could be recovered by dissolving the precipitate in 1 M maltose and precipitating alginate alone by addition of Ca2+. Based upon these observations, a-amylase from wheat germ was purified with 68-fold purification and 72% recovery. The molecular weight estimated by SDS–PAGE was 18 kDa. The method also worked equally well with a-amylase for the whole wheat seed. The latter enzyme could be purified 54- fold with 70% activity recovery. The molecular weight of this second enzyme was estimated to be 45 kDa by SDS–PAGE. | en |
| format.extent | 1049217 bytes | - |
| format.mimetype | application/pdf | - |
| language.iso | en | en |
| subject | amylase | en |
| subject | amylase from wheat germ | en |
| subject | purification | en |
| subject | wheat | en |
| title | Purification of wheat germ amylase by precipitation | en |
| type | Article | en |
| Appears in Collections: | Chemistry
|
Files in This Item:
| File |
Description |
Size | Format |
|---|
| sharmapur2000.pdf | | 1024Kb | Adobe PDF | View/Open |
|
Show simple item record
All items in DSpace are protected by copyright, with all rights reserved.
|
