DSpace
 

EPrints@IIT Delhi >
Faculty Research Publicatons  >
Chemistry >

Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/765

Full metadata record

DC FieldValueLanguage
dc.contributor.authorSharma, Aparna-
dc.contributor.authorSharma, Shweta-
dc.contributor.authorGupta, M N-
dc.date.accessioned2005-08-13T07:41:41Z-
dc.date.available2005-08-13T07:41:41Z-
dc.date.issued2000-
dc.identifier.citationProtein Expression and Purification, 18(1), 111–114en
dc.identifier.urihttp://eprint.iitd.ac.in/dspace/handle/2074/765-
dc.description.abstractAmylase from various sources was found to bind alginate in free solution. The alginate–enzyme complex could be precipitated with Ca2+. The enzyme activity could be recovered by dissolving the precipitate in 1 M maltose and precipitating alginate alone by addition of Ca2+. Based upon these observations, a-amylase from wheat germ was purified with 68-fold purification and 72% recovery. The molecular weight estimated by SDS–PAGE was 18 kDa. The method also worked equally well with a-amylase for the whole wheat seed. The latter enzyme could be purified 54- fold with 70% activity recovery. The molecular weight of this second enzyme was estimated to be 45 kDa by SDS–PAGE.en
dc.format.extent1049217 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoenen
dc.subjectamylaseen
dc.subjectamylase from wheat germen
dc.subjectpurificationen
dc.subjectwheaten
dc.titlePurification of wheat germ amylase by precipitationen
dc.typeArticleen
Appears in Collections:Chemistry

Files in This Item:

File Description SizeFormat
sharmapur2000.pdf1.02 MBAdobe PDFView/Open
View Statistics

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

 

Valid XHTML 1.0! DSpace Software Copyright © 2002-2010  Duraspace - Feedback