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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/856

Title: Separation of phospholipase D from peanut on a fluidized bed of crosslinked alginate beads
Authors: Sharma, Shweta
Roy, Ipsita
Gupta, Munishwar N
Keywords: Alginate
Phospholipase D
Fluidized beds
Issue Date: 2001
Citation: Biochemical Engineering Journal, 8(3), 235–239
Abstract: Alginate, a polysaccharide consisting of guluronic acid and mannuronic acid residues, has been crosslinked with epichlorohydrin to obtain beads. The crosslinked alginate beads were used in a fliudized bed to purify phospholipase D from peanut. The binding followed the Langmuir model. The maximum binding capacity (qm) for the enzyme was around 416Uml−1 and dissociation constant (Kd) was 217Uml−1. The enzyme could be purified (directly from the crude extract) 317-fold with 78% activity recovery. The results are compared with those obtained with a single plate process of affinity precipitation.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/856
Appears in Collections:Chemistry

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