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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/937

Title: Hydrolysis of rice hull by crosslinked Aspergillus niger cellulase
Authors: Sharma, A
Khare, S K
Gupta, M N
Keywords: Aspergillus niger
Cellulase
Crosslinking
Glutaraldehyde
Heat stable
Rice hull
Biomass
Saccharification
Enzymatic cellulose hydrolysis
Issue Date: 2001
Citation: Bioresource Technology, 78(3), 281-284
Abstract: A.niger cellulase was crosslinked by glutaraldehyde to obtain a heat-stable enzyme preparation for rice hull cellulose hydrolysis. Under optimized crosslinking conditions of 0.12 M glutaraldehyde, pH 7.0, temperature 40°C and at 45 min of crosslinking, a preparation having 15% more activity than free enzyme was obtained which also had considerable improvement in heat stability at 65°C and 70°C. Whereas the free enzyme lost 80% of its activity in 4 h at 65°C, the crosslinked preparation lost only 30% activity. The crosslinked preparation hydrolyzed cellulosic biomass more effectively giving 2.2 mg/ml glucose and 52% corresponding saccharification in 4 h at 65°C as compared to 14% saccharification by free enzyme under similar conditions.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/937
Appears in Collections:Chemistry

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