EPrints@IIT Delhi : Item 2074/937

Please use this identifier to cite or link to this item: http://hdl.handle.net/2074/937

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DC Field	Value	Language
contributor.author	Sharma, A	-
contributor.author	Khare, S K	-
contributor.author	Gupta, M N	-
date.accessioned	2005-10-18T05:45:58Z	-
date.available	2005-10-18T05:45:58Z	-
date.issued	2001	-
identifier.citation	Bioresource Technology, 78(3), 281-284	en
identifier.uri	http://eprint.iitd.ac.in/dspace/handle/2074/937	-
description.abstract	A.niger cellulase was crosslinked by glutaraldehyde to obtain a heat-stable enzyme preparation for rice hull cellulose hydrolysis. Under optimized crosslinking conditions of 0.12 M glutaraldehyde, pH 7.0, temperature 40°C and at 45 min of crosslinking, a preparation having 15% more activity than free enzyme was obtained which also had considerable improvement in heat stability at 65°C and 70°C. Whereas the free enzyme lost 80% of its activity in 4 h at 65°C, the crosslinked preparation lost only 30% activity. The crosslinked preparation hydrolyzed cellulosic biomass more effectively giving 2.2 mg/ml glucose and 52% corresponding saccharification in 4 h at 65°C as compared to 14% saccharification by free enzyme under similar conditions.	en
format.extent	361489 bytes	-
format.mimetype	application/pdf	-
language.iso	en	en
subject	Aspergillus niger	en
subject	Cellulase	en
subject	Crosslinking	en
subject	Glutaraldehyde	en
subject	Heat stable	en
subject	Rice hull	en
subject	Biomass	en
subject	Saccharification	en
subject	Enzymatic cellulose hydrolysis	en
title	Hydrolysis of rice hull by crosslinked Aspergillus niger cellulase	en
type	Article	en
Appears in Collections:	Chemistry

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