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http://hdl.handle.net/2074/940
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| Title: | Free polymeric bioligands in aqueous two-phase affinity extractions of microbial xylanases and pullulanase |
| Authors: | Teotia, Sunita
Lata, R
Gupta, M N |
| Keywords: | alginate
aqueous two-phase system
eudragit S-100
polyethylene glycol (PEG)
pullulanase
reversibly soluble-insoluble polymers
xylanase |
| Issue Date: | 2001 |
| Citation: | Protein Expression and Purification, 22(3), 484–488 |
| Abstract: | Two reversibly soluble-insoluble polymers (viz. Eudragit S-100 and alginate) were used as free macroaffinity bioligands in polyethylene glycol (PEG)/salt two-phase systems for separation of enzymes. Incorporation of Eudragit S-100 and alginate in the PEG phase led to considerable selectivity in separation of microbial xylanases and pullulanase, respectively. Xylanase from Aspergillus niger was recovered 93% with 56-fold purification, whereas the enzyme from Trichoderma reesei and Bacillus amyloliquefaciens was obtained with 93% activity recovery (31-fold purification) and 90% activity recovery (32-fold purification), respectively. From Bacillus acidopullulyticus pullulanase, 85% enzyme activity recovery with 44-fold purification was obtained. The approach described here shows the potential of developing into a general approach for use of reversibly soluble-insoluble macroaffinity ligand in two-phase affinity extraction. |
| URI: | http://eprint.iitd.ac.in/dspace/handle/2074/940 |
| Appears in Collections: | Chemistry
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