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dc.contributor.authorTeotia, Sunita-
dc.contributor.authorLata, R-
dc.contributor.authorGupta, M N-
dc.identifier.citationProtein Expression and Purification, 22(3), 484–488en
dc.description.abstractTwo reversibly soluble-insoluble polymers (viz. Eudragit S-100 and alginate) were used as free macroaffinity bioligands in polyethylene glycol (PEG)/salt two-phase systems for separation of enzymes. Incorporation of Eudragit S-100 and alginate in the PEG phase led to considerable selectivity in separation of microbial xylanases and pullulanase, respectively. Xylanase from Aspergillus niger was recovered 93% with 56-fold purification, whereas the enzyme from Trichoderma reesei and Bacillus amyloliquefaciens was obtained with 93% activity recovery (31-fold purification) and 90% activity recovery (32-fold purification), respectively. From Bacillus acidopullulyticus pullulanase, 85% enzyme activity recovery with 44-fold purification was obtained. The approach described here shows the potential of developing into a general approach for use of reversibly soluble-insoluble macroaffinity ligand in two-phase affinity extraction.en
dc.format.extent835926 bytes-
dc.subjectaqueous two-phase systemen
dc.subjecteudragit S-100en
dc.subjectpolyethylene glycol (PEG)en
dc.subjectreversibly soluble-insoluble polymersen
dc.titleFree polymeric bioligands in aqueous two-phase affinity extractions of microbial xylanases and pullulanaseen
Appears in Collections:Chemistry

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