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http://hdl.handle.net/2074/940
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| DC Field | Value | Language |
|---|
| contributor.author | Teotia, Sunita | - |
| contributor.author | Lata, R | - |
| contributor.author | Gupta, M N | - |
| date.accessioned | 2005-10-18T05:47:22Z | - |
| date.available | 2005-10-18T05:47:22Z | - |
| date.issued | 2001 | - |
| identifier.citation | Protein Expression and Purification, 22(3), 484–488 | en |
| identifier.uri | http://eprint.iitd.ac.in/dspace/handle/2074/940 | - |
| description.abstract | Two reversibly soluble-insoluble polymers (viz. Eudragit S-100 and alginate) were used as free macroaffinity bioligands in polyethylene glycol (PEG)/salt two-phase systems for separation of enzymes. Incorporation of Eudragit S-100 and alginate in the PEG phase led to considerable selectivity in separation of microbial xylanases and pullulanase, respectively. Xylanase from Aspergillus niger was recovered 93% with 56-fold purification, whereas the enzyme from Trichoderma reesei and Bacillus amyloliquefaciens was obtained with 93% activity recovery (31-fold purification) and 90% activity recovery (32-fold purification), respectively. From Bacillus acidopullulyticus pullulanase, 85% enzyme activity recovery with 44-fold purification was obtained. The approach described here shows the potential of developing into a general approach for use of reversibly soluble-insoluble macroaffinity ligand in two-phase affinity extraction. | en |
| format.extent | 835926 bytes | - |
| format.mimetype | application/pdf | - |
| language.iso | en | en |
| subject | alginate | en |
| subject | aqueous two-phase system | en |
| subject | eudragit S-100 | en |
| subject | polyethylene glycol (PEG) | en |
| subject | pullulanase | en |
| subject | reversibly soluble-insoluble polymers | en |
| subject | xylanase | en |
| title | Free polymeric bioligands in aqueous two-phase affinity extractions of microbial xylanases and pullulanase | en |
| type | Article | en |
| Appears in Collections: | Chemistry
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| teotiafre2001.pdf | | 816Kb | Adobe PDF | View/Open |
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