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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/963

Title: Purification of phospholipase D from dacus carota by three-phase partitioning and its characterization
Authors: Sharma, Shweta
Gupta, M N
Keywords: three-phase partitioning
phospholipase D
t-butanol
dacus carota
Issue Date: 2001
Citation: Protein Expression and Purification, 21(2), 310–316
Abstract: Phospholipase D from Dacus carota (carrot) was purified by subjecting it to three-phase partitioning. The single step of three phase partitioning led to 13-fold purification with an activity recovery of 72%. SDS- PAGE analysis showed a single band with minimum molecular weight corresponding to nearly 60 kDa. The purified enzyme had a pH optimum in the range of 6.0–6.5 and was unstable above 308C. Kinetic studies showed a Km value of 9.5 mM and a Vmax of 0.35 mL min21. The enzyme purified by three-phase parti- tioning was found to resolve into two isoenzymes on a DEAE-cellulose column.
URI: http://eprint.iitd.ac.in/dspace/handle/2074/963
Appears in Collections:Chemistry

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