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Please use this identifier to cite or link to this item: http://eprint.iitd.ac.in/handle/2074/963

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dc.contributor.authorSharma, Shweta-
dc.contributor.authorGupta, M N-
dc.identifier.citationProtein Expression and Purification, 21(2), 310–316en
dc.description.abstractPhospholipase D from Dacus carota (carrot) was purified by subjecting it to three-phase partitioning. The single step of three phase partitioning led to 13-fold purification with an activity recovery of 72%. SDS- PAGE analysis showed a single band with minimum molecular weight corresponding to nearly 60 kDa. The purified enzyme had a pH optimum in the range of 6.0–6.5 and was unstable above 308C. Kinetic studies showed a Km value of 9.5 mM and a Vmax of 0.35 mL min21. The enzyme purified by three-phase parti- tioning was found to resolve into two isoenzymes on a DEAE-cellulose column.en
dc.format.extent612303 bytes-
dc.subjectthree-phase partitioningen
dc.subjectphospholipase Den
dc.subjectdacus carotaen
dc.titlePurification of phospholipase D from dacus carota by three-phase partitioning and its characterizationen
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