भारतीय प्रौद्योगिकी संस्थान दिल्ली
Indian Institute of Technology, Delhi
  • Login
View Item 
  •   Home
  • Faculty Research Publications
  • Biochemical Engg. and Biotechnology
  • View Item
  •   Home
  • Faculty Research Publications
  • Biochemical Engg. and Biotechnology
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Protein fractionationin a vortex flow filter. II: Separation of simulated mixtures

Thumbnail
View/Open
balakrishnanpr95.pdf


Collections
  • Biochemical Engg. and Biotechnology [73]
Metadata
Show full item record
Author: Balakrishnan, M; Agarwal, G P

Advisor: Advisor

Date: 1996

Publisher: Elsevier S

Citation: Journal of

Series/Report no.:
Item Type:
Keywords: Ultrafiltration; Vortex flow filter; Protein transmission; Concentration polarization; Protein fractionation; Separation factor

Abstract: This work investigates the fractionation of simulated mixtures of lysozyme/ovalbumin and lysozyme/myoglobin in a vortex flow ultrafilter. The operating parameters for the separations were identified from transmission studies conducted previously for each of these three proteins [3]. At low transmembrane pressure and high membrane rotation speed, the ultrafiltration characteristics of a dilute protein mixture were virtually identical to those of its individual components. The selectivity was controlled primarily by the extent of polarization of the smaller, preferentially transported species (lysozyme). Thus, high separation factors were obtained under operating conditions favoring lysozyme buildup at the membrane surface. It was further observed that better separation occurred at low fluxes (corresponding to low applied pressure) thereby indicating that the throughput and degree of resolution are mutually exclusive.
Contact Us
Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
NewsClips @IITD
  • Facebook
  • twitter
  • youtube
  • instagram
  • pinterest
  • Linkedin

Browse

All of IITDCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister
Contact Us
Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
NewsClips @IITD
  • Facebook
  • twitter
  • youtube
  • instagram
  • pinterest
  • Linkedin