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Protein fractionation in a vortex flow filter. I: Effect of systemhydrodynamics and solution environment on single protein transmission

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Author: Balakrishnan, M; Agarwal, G P

Advisor: Advisor

Date: 1996

Publisher: Elsevier S

Citation: Journal of

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Keywords: Ultrafiltration; Vortex flow filter; Protein transmission; Module hydrodynamics; Solution environment

Abstract: Protein fractionation by ultrafiltration has elicited considerable interest in recent years. It is now recognised that a proper choice of the membrane and/or appropriate adjustment of operating conditions can successfully resolve binary protein mixtures. However, in order to identify the optimum conditions for selective filtration, it is essential to understand the UF characteristics of single proteins. In this paper, we have examined the flux and ransmission behavior of three different proteins, viz. lysozyme (13.93 kD, pl 10.6), ovalbumin (43.5 kD, pI 4.6) and myoglobin (16.89 kD, pI 6.8) as a function of operating variables in a vortex flow filter using 100 kD hydrophilic polyacrylonitrile membranes. The effects of both the module hydrodynamics, i.e. transmembrane pressure, axial velocity and rotation speed as well as the solution environment, i.e. protein concentration, ionic strength and pH were investigated. It was determined that ydrodynamics is primarily controlled by the transmembrane pressure and the membrane rotation rate. Also, variations in the feed solution properties, particularly the ionic strength and pH could dramatically alter the protein ransmission profiles. These results provide a basic framework for designing effective lysozyme/ovalbumin and lysozyme/myoglobin separations.
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Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
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