भारतीय प्रौद्योगिकी संस्थान दिल्ली
Indian Institute of Technology, Delhi
  • Login
View Item 
  •   Home
  • Faculty Research Publications
  • Biochemical Engg. and Biotechnology
  • View Item
  •   Home
  • Faculty Research Publications
  • Biochemical Engg. and Biotechnology
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Effects of dissolved oxygen and oxygen mass transfer on overexpression of target gene in recombinant E. coli

Thumbnail
View/Open
bhattacharyaeff97.pdf


Collections
  • Biochemical Engg. and Biotechnology [73]
Metadata
Show full item record
Author: Bhattacharya, Sanjoy K; Dubey, Ashok K

Advisor: Advisor

Date: 1997

Publisher:
Citation: Enzyme and

Series/Report no.:
Item Type: Article

Keywords: Recombinant E. coli; IFTG induction; Bioreactor; Dissolved oxygen level; Oxygen mass transfer; Expression of cloned gene

Abstract: Overexpression of target gene (Mspl methylase) in recombinant Escherichia coli has been studied in response to dissolved oxygen and oxygen mass transfer. The rate of target gene expression attained its maximum value (2.67 U mg-1p-1h-1) when the culture was induced with 1 llzM isopropyl+-o-thiogalactopyranoside (IPTG) in the mid-exponential phase of growth. The maximum value of 6.7 x ld U mg-1p-1 for the target gene expression was attained in about 3 h ofpostinduction cultivation. An oxygen-suficient condition (0.22 mmoles I-1) was necessary to obtain optimal expression and cell productivity. Expression of the target gene upon induction was accompa-nied by an increase in oxygen uptake rate (OUR) of the cells and decrease in dissolved oxygen (DO) concen- tration in the cultivation broth. Volumetric oxygen transfer coeficient (K,a) of 61 h-1 was optimum for cell productivity of uninduced culture while that for induced culture was 84 h-1 which was optimum for foreign protein synthesis also.
Contact Us
Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
NewsClips @IITD
  • Facebook
  • twitter
  • youtube
  • instagram
  • pinterest
  • Linkedin

Browse

All of IITDCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister
Contact Us
Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
NewsClips @IITD
  • Facebook
  • twitter
  • youtube
  • instagram
  • pinterest
  • Linkedin