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Productivity enhancement in L-sorbose fermentation using oxygen vector

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Author: Giridhar, R; Srivastava, A K

Advisor: Advisor

Date: 2000

Publisher:
Citation: Enzyme and

Series/Report no.:
Item Type: Article

Keywords: Vitamin-C; Acetobacter suboxydans; D-sorbitol; L-sorbose; Fed-batch fermentation; Oxygen vector

Abstract: Sorbose, an intermediate of Vitamin-C is produced by biological oxidation of sorbitol by Acetobacter suboxydans. The utility of oxygen vector (N-hexadecane) in enhancing the sorbose accumulation and its productivity was examined for sorbitol to sorbose bioconversion process. Shake flask fermentations were conducted using 1% to 6% (v/v) N-hexadecane. Higher sorbose production was observed in shake flask containing 1–4% N-hexadecane as compared to shake flasks without N-hexadecane. A maximum of 82.12 kgm23 sorbose accumulated in 24 h by the addition of 4% N-hexadecane as against 64.83 kgm23 sorbose without the addition of N-hexadecane. However, the concentration of sorbose produced in the same time decreased when 5% and 6% N-hexadecane were used. Batch sorbose fermentation conducted using 4% N-hexadecane demonstrated decrease in the process time from 14 h to 12 h. The productivity increased from 14.3 kgm23 h21 to 16.7 kgm23 h21 when 4% N-hexadecane was used. Fed-batch fermentation using 4% N-hexadecane was over in 20 h with a productivity of 15.9 kgm23 h21, and a sorbose accumulation of 311.68 kgm23 was obtained. The same fed-batch fermentation finished in 25 h when no N-hexadecane was used. A productivity of 12.6 kgm23 h21 and a final sorbose accumulation of 316 kgm23 were obtained.The increase in productivity and lower process time were achieved by the addition of N-hexadecane to the fermentation medium.
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Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
NewsClips @IITD
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