Author: | Seth, Malini; Chand, Subhash |
Advisor: | Advisor |
Date: | 2000
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Publisher: | |
Citation: | Process Bi
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Series/Report no.: |
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Item Type: | Article
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Keywords: | Tannase biosynthesis; Gallic acid accumulation; Optimisation; Response surface method; Aspergillus awamori |
Abstract: | Tannin acyl hydrolase (tannase) is an industrially important enzyme produced by a large number of fungi. Tannic acid
concentration, agitation speed and pH during the fermentation were identified as important process parameters effecting cell
growth and enzyme synthesis by Aspergillus awamori. These parameters were optimised in a laboratory bioreactor by response
surface methodology using Box and Behnken factorial design to determine the optimum conditions for enzyme production and
gallic acid accumulation. Under optimum process conditions for enzyme synthesis, the fermentation run lasted 60 h with an initial tannic acid concentration of 35.0 g l, yielding biomass concentration of 7.13 g l containing 771 IU of intracellular tannase per gram dry cell weight and 19 g l of gallic acid. However, maximum gallic acid accumulation (40.3 g l) was obtained in
24 h with an initial substrate concentration of 45 g l. |