Author: | Giridhar, R; Srivastava, Ashok K |
Advisor: | Advisor |
Date: | 2001
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Publisher: | |
Citation: | Process Bi
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Series/Report no.: |
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Item Type: | Article
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Keywords: | Acetobacter suboxydans; Fed-batch fermentation; Vitamin C; Sorbitol; Sorbose; Feeding strategy |
Abstract: | L-sorbose fermentation is of industrial importance since it is an intermediate step in the industrial production of vitamin C. It involves the biological oxidation of D-sorbitol to L-sorbose using Acetobacter suboxydans. Batch sorbose fermentation features substrate inhibition and hence it is impossible to obtain high sorbose productivity in batch bioreactors. Fed-batch fermentations can eliminate substrate inhibition and improve sorbose productivity. Fed-batch sorbose fermentations were conducted using two
different nutrient feeding strategies. Fed-batch fermentation conducted by feeding nutrients containing 600 g1 of sorbitol at a
constant feed rate of 0.6 l yielded a sorbose productivity of 12.64 g1-h. While fed-batch fermentation conducted by
feeding nutrients containing 600 g1 of sorbitol at a linearly decreasing feed rate (0.5–0.041 h) demonstrated a productivity
of 17.01 g1 h. The productivity obtained by feeding nutrients at a linearly decreasing feed rate was higher than that obtained
by the constant fed-batch fermentation and, therefore, is a better substrate feeding strategy for improving sorbose productivity. |