भारतीय प्रौद्योगिकी संस्थान दिल्ली
Indian Institute of Technology, Delhi
  • Login
View Item 
  •   Home
  • Faculty Research Publications
  • Biochemical Engg. and Biotechnology
  • View Item
  •   Home
  • Faculty Research Publications
  • Biochemical Engg. and Biotechnology
  • View Item
JavaScript is disabled for your browser. Some features of this site may not work without it.

Thermal stability of alcohol dehydrogenase enzyme determined by activity assay and calorimetry

Thumbnail
View/Open
naththe98.pdf


Collections
  • Biochemical Engg. and Biotechnology [73]
Metadata
Show full item record
Author: Nath, Sunil; Satpathy, Gyana R; Mantri, Rahul; Deep, Shashank; Ahluwalia, Jagdish C

Advisor: Advisor

Date: 1998

Publisher:
Citation: Thermochim

Series/Report no.:
Item Type: Article

Keywords: Activity assay; Alcohol dehydrogenase; Calorimetry; Denaturation enthalpy; Denaturation temperature; Kinetic parameters; Thermal stability

Abstract: The thermostability of pure yeast alcohol dehydrogenase was investigated at various temperatures, in the presence and absence of sucrose, by both activity assay and differential scanning calorimetry. The thermal inactivation exhibited nonlinear blphasic behavior. The thermal inactivation rate constants and the magnitude of the heat-stable and heat-labile fractions of the enzyme were quantified. The values of the denaturation temperature were experimentally measured by calorimetry. It was found that although activity assay and calorimetry are based on different principles, they yield results that agree well with each other. However, each technique provides unique data (e.g. enzyme activity vis-a-vis basic thermodynamic properties, such as the denaturation enthalpy) and the two techniques may be considered complementary to each other.
Contact Us
Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
NewsClips @IITD
  • Facebook
  • twitter
  • youtube
  • instagram
  • pinterest
  • Linkedin

Browse

All of IITDCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

My Account

LoginRegister
Contact Us
Shankar B. Chavan
Computer Applications Division
Central Library, IIT Delhi
shankar.chavan@library.iitd.ac.in
NDLTD
Shodhganga
NDL
ePrints@IISc
etd@IISc
IR@IIT Bombay
NewsClips @IITD
  • Facebook
  • twitter
  • youtube
  • instagram
  • pinterest
  • Linkedin